Screening thermotolerant white-rot fungi for decolorization of wastewaters |
| |
Authors: | P Chairattanamanokorn T Imai R Kondo M Ukita P Prasertsan |
| |
Institution: | (1) Department of Civil and Environmental Engineering, Yamaguchi University, Tokiwadai 2-16-1, 755-8611 Yamaguchi, Japan;(2) Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 812-8581 Fukuoka, Japan;(3) Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, 90112 Songkla, Thailand |
| |
Abstract: | To select a thermotolerant fungal strain for decolorization of wastewaters, ligninolytic enzyme production (lignin peroxidase,
manganese peroxidase MnP], and laccase), decolorization, and removal of total phenol and chemical oxygen demand (COD) were
detected. Thirty-eight fungal strains were studied for enzyme production at 35 and 43°C on modified Kirk agar medium including
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and MnCl2. Thirteen strains grew on manganese-containing agar and provided green color on ABTS-containing agar plates under culture
at 43°C. Decolorization of wastewater from alcohol distillery (WAD) by these strains was compared under static culture at
43°C, and Pycnoporus coccineus FPF 97091303 showed the highest potential. Thereafter, immobilized mycelia were compared with free mycelia for WAD decolorization
under culture conditions of 43°C and 100 rpm. The immobilized mycelia on polyurethane foam enhanced the ligninolytic enzyme
production as well as total phenol and color removal. At about the same COD removal, MnP and laccase produced by immobilized
mycelia were 2 and 19 times higher than by free mycelia; the simultaneous total phenol and color removal were 3.1 and 1.5
times higher than the latter. Moreover, decolorization of synthesis dye wastewater was carried out at 43°C and 100 rpm. More
than 80% of 300 mg/L of reactive blue-5 was decolorized by the immobilized mycelia within 1 to 2 d for four cycles. |
| |
Keywords: | Thermotolerant fungi Pycnoporus coccineus ligninolytic enzyme decolorization screening |
本文献已被 PubMed SpringerLink 等数据库收录! |
|