Detection of magnetic-labeled antibody specific recognition events by combined atomic force and magnetic force microscopy |
| |
| Authors: | Xia Hong Yanmei Liu Wei Guo |
| |
| Affiliation: | a Center for Advanced Optoelectronic Functional Materials Research, Key Laboratory of UV Light-Emitting Materials and Technology, Ministry of Education, Northeast Normal University, Changchun 130024, PR China
b College of Chemistry, Jilin University, Changchun 130023, PR China |
| |
| Abstract: | Atomic force (AFM) and magnetic force microscopy (MFM) were developed to detect biomolecular specific interaction. Goat anti-mouse immunoglobulin (anti-IgG) was covalently attached onto gold substrate modified by a self-assembly monolayer of thioctic acid via 1-ethyl-3-[3-(dimethylamino) propyl] carbodiimide (EDC) activation. Magnetic-labeled IgG then specifically adsorbed onto anti-IgG surface. The morphological variation was identified by AFM. MFM was proved to be a fine assistant tool to distinguish the immunorecognized nanocomposites from the impurities by detection of the magnetic signal from magnetic-labeled IgG. It would enhance the understanding of biomolecular recognition process. |
| |
| Keywords: | AFM MFM Magnetic label Specific recognition Antibody/anti-IgG |
| 本文献已被 ScienceDirect 等数据库收录！ |
