Magnetic iron particles with high magnetization useful for immunoassay |
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Authors: | Hisato Tokoro Takashi Nakabayashi Hong Zhao |
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Institution: | a Hitachi Metals, Ltd., Advanced Electronics Research Laboratory, 5200 Mikajiri, Kumagaya, Saitama 360-0843, Japan b Faculty of Pharmaceutical Sciences, University of British Columbia, 2146 East Mall, Vancouver, Canada BC V6T 1Z3 |
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Abstract: | TiO2-encapsulated metallic Fe particles (Ti-O/Fe) were synthesized through a solid phase reaction. The Ti-O/Fe particles were non-toxic to tumor cells in a cell viability assay. After silica coating using a sol-gel method, streptavidin was covalently bound onto the Ti-O/Fe particles. Thus produced HMMI particles showed higher magnetization (114 Am2/kg) and a larger specific surface area (15 m2/g) than conventional streptavidin-immobilized magnetite particles. The high magnetization allowed for rapid magnetic separation, while the additional large specific surface area improved the detection of the adiponectin antigen both in terms of extended detection range and higher assay speed. |
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Keywords: | Metallic Fe particle High magnetization Non-toxicity Rapid magnetic separation Sensitive immunoassay |
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