Chelerythrine chloride binding of guanosine in aqueous solution

In this work, the interaction between chelerythrine (CHE) and guanosine is studied usingUV-vis and fluorescence measurements at various temperatures. The UV-vis spectra show that the increasing guanosine concentrations result in the decreasing absorption intensity and red shift of CHE E absorption band (267 nm). The fluorescence spectra are fitted to linear analysis, yielding a binding constant of 1.04×10⁴ L/mol at 298.15 K of CHE with guanosine. Besides, with △_rH_m^Θ=-8.26 kJ=mol, △_rG_m^Θ=-22.90 kJ=mol,and △_rS_m^Θ=-49.38 J/(molK)the interaction should be entropy-driven and exterothermic.