Validation of a strategic approach to high-performance liquid chromatographic method selection |
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Authors: | T. Hamoir B. Bourguignon D. L. Massart |
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Affiliation: | (1) Pharmaceutical Institute, Vrije Universiteit Brussels, Laarbeeklaan 103, 1090 Brussels, Belgium |
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Abstract: | Summary A recently reported chromatographic method selection strategy has been validated using fifteen drug formulations selected randomly from the Belgium Compendium, 1992. On the basis of the hydrophobic and the acidic — basic properties of the sample components, reversed-phase was recommended as the first choice mode for all formulations. For two multicomponent formulations the range of analyte polarity dictated the need for gradient elution. The commerical software DryLab G/plus® was used for selection of optimum gradient conditions. The results obtained by both isocratic and gradient chromatography are discussed, as is the usefulness of a tailing suppressor in both modes. |
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Keywords: | Column liquid chromatography First-guess Retention optimization Validation |
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