电感耦合等离子体发射光谱法测定食用菌中微量元素的方法研究

采用高压硝化罐在较低温度下进行缓慢样品消化,减少了消化过程中微量元素的损失。采用电感耦合等离子体发射光谱法测定了食用菌中Ca,Na,K,Mg,Mn,Ba,Fe,Co,Ge和Cu等多种微量元素的含量。高压硝化罐法测定各元素的相对标准偏差在0·16%～2·86%之间,湿法测定各元素的相对标准偏差在0·33%～3·49%之间。可见高压硝化罐法处理样品的测量精密度和湿法处理样品的测量精密度均比较好,前者的测量精密度更高一些。对高压硝化罐法和湿法处理样品的测量结果进行了t检验,t值在0·0024～2·473,均小于t0·99,9(3·25),说明两种方法的测量结果无显著性差异,即两种方法不存在系统误差。同时,高压硝化罐法处理样品的回收率在96·6%～103%之间,该方法具有简便、快速、灵敏、稳定、准确等优点,适于食用菌中微量元素的分析测定。

Determination of Trace Elements in the Edible Fungi by Inductively Coupled Plasma Atomic Emission Spectrometry

In the present paper,samples were digested by high pressure digestion pots,reducing the loss of trace elements during the digestion process.The contents of trace elements in edible fungi such as Ca,Na,K,Mg,Mn,Ba,Fe,Co,Ge and Cu were determined by inductively coupled plasma atomic emission spectrometry.The relative standard deviation with high pressure digestion pots was 0.16%-2.86%,and that with wet method was 0.33%-3.49%,so it could be seen that the measurement precisions of the two methods were good,and the former was better.The measurement results with the two methods were checked by t-test,and the values in the range of 0.002 4-2.473 were lower than t0.99,9(3.25).The measurement results showed that the two methods had no obvious differences,namely no system errors occurred.The recovery rates with high pressure digestion pots were in the range of 96.6%-103%.The method of high pressure digestion pots was suitable for the determination of trace elements in edible fungi with the advantages of being simple,rapid,sensitive,stable and accurate etc.,and the results were satisfactory.