Preparation and Porous Property of C14-Monolithic Column for Capillary Electrochromatography |
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| Authors: | Kai Zhang Ruyu Gao Chao Yan Zhichao Zhang Qinsun Wang |
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| Institution: | (1) State Key Laboratory of Elemento-Organic Chemistry, Nankai University, 94 Weijin Road, Tianjin, 300071, China;(2) Unimicro Technologies, Inc., 4713 First Street, Pleasanton, CA 94566, USA;(3) Present address: Laboratory of Electrochemistry and Analytical Chemistry, UMR CNRS 7575-ENSCP, 11 Rue Pierre et Marie Curie, 75231 Paris Cedex 05, France |
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| Abstract: | Capillary electrochromatography (CEC) has been performed with a series of C14, methlyacrylamide based monolithic columns. These monoliths with different porosities were prepared by in-situ copolymerization in fused-silica capillaries. The porous properties of monoliths were further observed using scanning electron micrographs (SEM) and measured using a mercury porosimeter. The effect of various alcohols as porogens on porous structural properties and chromatographic behaviors were also investigated. The effects of organic additive, pH value and ionic strength in mobile phase on electroosmosis flow (EOF) and separation were further discussed. Meanwhile, the baseline separation of 6 neutral compounds can be well obtained. In addition, the monolithic column demonstrates the high column efficiency and satisfactory reproducibility.Acknowledgements We gratefully acknowledge the support of the National Natural Science Foundation of China. |
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| Keywords: | Capillary electrochromatography Monolithic columns Porogens |
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