| 两种带有不同间隔臂的环糊精键合固定相保留行为的评价 |
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| 作者姓名： | Zhao Y Guo Z Xue X Liang X |
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| 作者单位： | 1. 中国科学院大连化学物理研究所1803组
2. 中国科学院大连化学物理研究所 |
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| 基金项目： | 国家自然科学基金项目(20975100,30801513,21005077) |
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| 摘 要： | 选取14种模型化合物对两种带有不同间隔臂的环糊精键合固定相(Click Alkyl-CD、Click OEG-CD)进行了反相液相色谱模式下的保留行为评价。通过梯度洗脱条件下保留参数计算方法和CSASS软件,根据3次线性梯度的保留值数据,测出14种溶质分子在两种固定相上的保留参数,在此基础上考察流动相含乙腈浓度与保留因子的关系后发现,Click Alkyl-CD和Click OEG-CD在分离非极性和中等极性化合物时主要基于反相液相色谱模式,而某些化合物(如吲唑)在Click OEG-CD上的保留受多种作用力影响,并不基于反相液相色谱模式。疏水性评价结果表明,反相分离模式下Click Alkyl-CD的保留参数和正辛醇-水分配常数的相关性较好(R=0.7),说明其具有比较强的疏水性;而Click OEG-CD的相关性不高(R＜0.3),说明疏水作用力以外的其他作用力对化合物在反相模式下的保留影响较大。
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| 关 键 词： | 环糊精固定相 液相色谱 保留行为 评价 |
| 收稿时间： | 2011-08-23 |
Evaluation of the retention properties of two cyclodextrin stationary phases with different spacers |
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| Zhao Y,Guo Z,Xue X,Liang X.Evaluation of the retention properties of two cyclodextrin stationary phases with different spacers[J].Chinese Journal of Chromatography,2011,29(9):885-889. |
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| Authors: | Zhao Yanyan Guo Zhimou Xue Xingya Liang Xinmiao |
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| Institution: | 1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. College of Pharmacy, Dalian Medical University, Dalian 116044, China |
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| Abstract: | A set of 14 model compounds were selected to evaluate the retention properties of two silica-based cyclodextrin(CD) bonded stationary phases with different spacers(Click Alkyl-CD and Click OEG-CD) under reversed-phase liquid chromatographic(RPLC) mode.The gradient calculation method and CSASS software developed in our group were applied to obtain the retention parameters of the 14 solutes on the two CD bonded stationary phases under 3 gradient conditions.The effect of acetonitrile content in the mobile phas... |
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| Keywords: | cyclodextrin(CD) stationary phase liquid chromatography retention property evaluation |
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