Localized surface plasmon resonance interfaces coated with poly[3-(pyrrolyl)carboxylic acid] for histidine-tagged peptide sensing |
| |
Authors: | Tighilt Fatma-Zohra Subramanian Palaniappan Belhaneche-Bensemra Naima Boukherroub Rabah Gabouze Noureddine Sam Sabrina Szunerits Sabine |
| |
Affiliation: | UDTS, 2, Bd Frantz-Fanon, B.P. 140 Alger-7 merveilles, 16200 Algiers, Algeria. |
| |
Abstract: | The paper reports on a novel localized surface plasmon resonance (LSPR) substrate architecture for the immobilization and detection of histidine-tagged peptides. The LSPR interface consists of an ITO (indium tin oxide) substrate coated with gold nanostructures. The latter are obtained by thermal deposition of a thin (2 nm thick) gold film followed by post-annealing at 500 °C. The LSPR interface was coated with poly[3-(pyrrolyl)carboxylic acid] thin films using electrochemical means. The ability of the LSPR interfaces coated with poly[3-(pyrrolyl)carboxylic acid] to chelate copper ions was investigated. Once loaded with metal ions, the modified LSPR interface was able to bind specifically to histidine-tagged peptides. The binding process was followed using LSPR. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|