| 蜗牛肝超氧化物歧化酶的纯化及性质研究 |
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| 引用本文： | 王转花,李改平.蜗牛肝超氧化物歧化酶的纯化及性质研究[J].山西大学学报(自然科学版),1994,17(4):407-410. |
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| 作者姓名： | 王转花 李改平 |
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| 作者单位： | 山西大学生命科学系，山西省中医学校 |
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| 摘 要： | 实验以新鲜蜗牛肝脏为材料，经匀浆、盐析、氯仿－乙醇处理、透析和ＳｅｐｈａｄｅｘＧ－７５柱层析等步骤、纯化得到超氧化物歧化酶。对其理化性质鉴定表明，用此法纯化的ＳＯＤ纯度均一，比活力为６８００Ｕ／ｍｇ·ｐｒ，分子量为３０．３ＫＤ，紫外最大吸收峰２５９．６ｎｍ，Ｎ－末端分析表明为Ａｌａ。
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| 关 键 词： | 超氧化物歧化酶，蜗牛肝，性质，纯化 |
PURIFICATION AND CHARACTERIZAION OF SUPEROXIDE DISMUTASE FROM SNAIL LIVER |
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| Wang Zhuanhua,Tian Qiaoyun.PURIFICATION AND CHARACTERIZAION OF SUPEROXIDE DISMUTASE FROM SNAIL LIVER[J].Journal of Shanxi University (Natural Science Edition),1994,17(4):407-410. |
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| Authors: | Wang Zhuanhua Tian Qiaoyun |
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| Abstract: | Superoxide dismutase was isolated and purified from snail liver by salting out, chloro-form-ethanol treatment dialysis and Sephadex G-75 column chromatography. The investiga-tion of physiochcmical properties shows that the purified SOD is homogenously pure,withthe specific activity being 6800U/mg pr.and molecular weight about 30.3KD.A maximumabsorption is found at 259.6nm,whose N-terminal amino acid is alanine. |
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| Keywords: | Superoxide dismutase characterization snail liver purification |
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