Superposition of permeation and adsorption effects in liquid chromatography

Summary A generalized treatment of the superposition of separations based on gel-permeation chromatography (GPC) and liquid-solid chromatography (LSC) is presented. Special emphasis is given to the fact that restricted-pore entry behaviour in GPC automatically eliminates part of the surface accessible for adsorption in LSC. Therefore the simple additivity rule for K_GPC and K_ads, applied so far exclusively to adsorptive GPC interpretation, cannot be used in general and special care has to be taken if information about adsorptivities is to be deduced from the chromatographic experiment.