Stiffness,resilience, compressibility |
| |
Authors: | Bogdan?M?Leu Email authorView authors OrcID profile" target="_blank">J?Timothy?SageEmail authorView authors OrcID profile |
| |
Institution: | 1.Advanced Photon Source,Argonne National Laboratory,Argonne,USA;2.Department of Physics and Center for Interdisciplinary Research on Complex Systems,Northeastern University,Boston,USA |
| |
Abstract: | The flexibility of a protein is an important component of its functionality. We use nuclear resonance vibrational spectroscopy (NRVS) to quantify the flexibility of the heme iron environment in the electron-carrying protein cytochrome c by measuring the stiffness and the resilience. These quantities are sensitive to structural differences between the active sites of different proteins, as illustrated by a comparative analysis with myoglobin. The elasticity of the entire protein, on the other hand, can be probed quantitatively from NRVS and high energy-resolution inelastic X-ray scattering (IXS) measurements, an approach that we used to extract the bulk modulus of cytochrome c. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|