Microchip isoelectric focusing using a miniature scanning detection system |
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Authors: | Raisi F Belgrader P Borkholder D A Herr A E Kintz G J Pourhamadi F Taylor M T Northrup M A |
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Affiliation: | Cepheid, Sunnyvale, CA 98089, USA. |
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Abstract: | A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric focusing (mIEF) analysis. The detector, comprised of a lamp and photomultiplier tube (PMT) on a moving stage, measured the real-time distribution of fluorescently labeled peptides subjected to gel-free mIEF. During the run, the effective length of the 6-cm channel was scanned every 9 s. Analysis was completed within 5 min while still obtaining high resolution and sensitivity. In addition, the scanning detector was used to characterize peptide migration properties within the channel by providing simultaneous temporal and spatial measurements. |
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