| PAN-蛋白质络合物的极谱吸附波及其应用 |
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| 引用本文： | 郑石英,罗登柏.PAN-蛋白质络合物的极谱吸附波及其应用[J].分析试验室,2010,29(3). |
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| 作者姓名： | 郑石英 罗登柏 |
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| 作者单位： | 1. 福建农林大学生命科学学院,福州,350002
2. 中南民族大学化学与材料科学学院,武汉,430074 |
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| 基金项目： | 湖北省自然科学基金(2005ABA001);;福建农林大学校青年基金(08B11)项目资助 |
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| 摘 要： | 在0.05 mol/L HAc溶液中,PAN-牛血清白蛋白/人血清白蛋白络合物在-0.58 V(vs.SCE)处产生一灵敏吸附还原峰,峰电位较之游离PAN还原峰负约0.28 V,峰电流与牛血清白蛋白、人血清白蛋白浓度在0.1～12 mg/L,0.1～11 mg/L范围内呈线性关系;检测限均为0.05 mg/L。运用该法测定了人血清样品蛋白质含量。
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| 关 键 词： | 单扫描极谱法 1-（2-吡啶偶氮）-2-萘酚（PAN） 牛血清白蛋白（BSA） 人血清白蛋白（HSA）络合物 |
A polargraphic absorption wave of PAN-proteins complex and its application |
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| ZHENG Shi-ying,LUO Deng-bai.A polargraphic absorption wave of PAN-proteins complex and its application[J].Chinese Journal of Analysis Laboratory,2010,29(3). |
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| Authors: | ZHENG Shi-ying LUO Deng-bai |
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| Institution: | 1.College of Life Sciences;Fujian Agriculture and Forestry University;Fuzhou 350002;2.College of Chemistry and Material Science;South Central University for Nationalities;Wuhan 430074 |
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| Abstract: | A sensitive reduction wave of PAN-proteins at -0.58 V (vs. SCE)has been observed by using single sweep polarography in 0.05 mol/L acetic acid and 7.5×10~(-6)mol/L PAN solution. The peak potential is 0.28 V more negative than that of PAN. The second order derivative peak height is linearly proportional to the concentration of BSA from 0.1 to 12 mg/L and to the concentration of HSA from 0.1 to 11 mg/L. The detection limit is 0.05 mg/L both for BSA and for HSA. The method has been used for the determination of total protein in human serum samples. The result is satisfactory. |
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| Keywords: | Single sweep polarography 1-(2-Pyridylazo)-2-naphthol (PAN) Bovine serum albumin (BSA) Human serum albumin (HSA) Complex |
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