高效毛细管电泳-安培检测研究核苷酸水解反应

核酸是重要的生命物质,近些年来,对核酸水解作用的研究已成为人们关注的焦点,寻找一种在温和的生理条件下能够迅速催化水解核酸的人工酶,对于进一步探索核酸的结构及其在生命活动中的作用,阐明细胞间信息传递的机理,以及对于抗癌药物的研制等具有重要意义.有关稀土金属离子及其配合物对核酸的催化作用已有报道1～3],这种选择性的水解作用断裂点单一,无碎片产生,克服了以往通过氧化作用断裂核酸带来的弊端4～5],但其作用的机理、水解体系的优化尚需进一步的探讨.以往对核酸水解作用的研究,主要是通过高效液相色谱(HPLC)1,6]、化学滴定和…

The Study on Hydrolysis of Nucleotides by High Performance Capillary Electrophoresis with Amperometric Detection

High performance capillary electrophoresis with amperometric detection was applied to the study on hydrolysis of adenosine monophosphate(AMP) in this article. Under the optimal conditions of electrophoresis(CTAB 0.2 mmol/L+PBS 50 mmol/L, pH=8.2), the factors affecting the hydrolysis of AMP and mechanism of the hydrolysis were investigated. The results showed that the analytical method introduced was fast, convenient and efficient. WT5HZ]