Purification and monolayer study of the thylacoid lipids of moss marchantia polymorpha

The original lipid content of the thylakoid membranes of moss Marchantia polymorpha has been determined for the first time. In particular, the content of SQDG is almost 3 times higher than those for both of the other classes. The ratios for DGDG and MGDG are just a little bit lower than those for green algae, but almost 2 times less than those for plants. The distribution of unsaturated bonds has changed in C₁₈ -residues of fatty acids. The total content of C₁₈-residues in thylacoid lipids have been almost the same but the content of C_18:1, C_18:2 and C_18:3 are altered in the fractions of DGDG, PG and PE. The light stress produces only the quantitative, but no qualitative, changes of the thylakoid lipid composition. The properties of the thylakoid lipids and corresponding fatty acids in the monolayers at the liquid/gas interfaces have been studied. The changes in distribution of unsaturated bonds in C₁₈ residues of fatty acids at light stress have been confirmed by Langmuir method.