| 牛血清白蛋白与纳米银的相互作用及半胱氨酸的检测 |
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| 引用本文： | 陈述,白珊,王贞,张萍萍,龙云飞. 牛血清白蛋白与纳米银的相互作用及半胱氨酸的检测[J]. 光谱实验室, 2011, 28(4): 2047-2049 |
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| 作者姓名： | 陈述 白珊 王贞 张萍萍 龙云飞 |
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| 作者单位： | 湖南科技大学化学化工学院 湖南省湘潭市桃园路1号411201 |
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| 基金项目： | 湖南省自然科学基金，湖南省青年骨干教师培养项目 |
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| 摘 要： | 表面无保护剂的小粒径纳米银与牛血清白蛋白（BSA）的相互作用可引起BSA特征荧光的猝灭。而若先向体系中加入半胱氨酸（Cys）,其巯基与A g的强相互作用可降低体系中的纳米银与BSA的相互作用,使BSA荧光猝灭程度降低。体系在350nm处荧光强度的变化与Cys的浓度呈线性关系,线性范围为3.3×10-8—3.3×10-4m ol/L,相关系数为0.9982。基于这种光谱的变化,可用来测定Cys。
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| 关 键 词： | 纳米银 荧光猝灭 牛血清白蛋白 半胱氨酸 |
Interaction Between Bovine Serum Albumin and Nanosilver and Determination of Cysteine |
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| CHEN Shu,BAI Shan,WANG Zhen,ZHANG Ping-Ping,LONG Yun-Fei. Interaction Between Bovine Serum Albumin and Nanosilver and Determination of Cysteine[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(4): 2047-2049 |
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| Authors: | CHEN Shu BAI Shan WANG Zhen ZHANG Ping-Ping LONG Yun-Fei |
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| Affiliation: | (School of Chemistry and Chemical Engineering,Hunan University of Science and Technology,Xiangtan,Hunan 411201,P.R.China) |
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| Abstract: | The interaction between bovine serum albumin（BSA） and small grain size nanosilver without protective agent was studied,which presented a quenching characteristic fluorescence of BSA.If cysteine was added into the system in advance,its mercapto groups can strongly interact with Ag,and the interaction of BSA and nanosilver decreased.The degrees of fluorescence quencheing could depressed.The fluorescence intensity had a linear relationship with the concentration of cysteine at 350nm,and the linear range was in the range of 3.3×10^-8mol/L and 3.3×10^-4mol/L with the correlation coefficient of 0.9982.Based on above the changes of spectral behaviors,the determination of the cysteine could be realized. |
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| Keywords: | Nanosiloer Fluorescence Quenching BAS Cysteine |
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