| 荧光猝灭法研究单羰基姜黄素衍生物与酪氨酸酶间的相互作用 |
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| 引用本文： | 徐学涛,张瑞瑞,杜志云,卢宇靖,张焜.荧光猝灭法研究单羰基姜黄素衍生物与酪氨酸酶间的相互作用[J].光谱实验室,2011,28(4):1862-1865. |
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| 作者姓名： | 徐学涛 张瑞瑞 杜志云 卢宇靖 张焜 |
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| 作者单位： | 广东工业大学轻工化工学院 广州市广州大学城外环西路100号510006 |
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| 摘 要： | 用二烯酮结构代替不稳定的二烯二酮结构合成了3个结构稳定的单羰基姜黄素衍生物1,5-双(4-羟基)-1,4-戊二烯-3-酮(1a)、2,5-双(4-羟基-苯亚甲基)环戊酮(1b)和2,6-双(4-羟基-苯亚甲基)环己酮(1c).运用荧光光谱法研究了化合物与酪氨酸酶间的相互作用.实验结果表明,酪氨酸酶可致使合成化合物的荧光...
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| 关 键 词： | 二烯酮 酪氨酸酶 荧光光谱法 |
Interaction Between Curcumin Derivant and Tyrosinase by Fluorescence Quenching Method |
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| XU Xue-Tao,ZHANG Rui-Rui,DU Zhi-Yun,LU Yu-Jing,ZHANG Kun.Interaction Between Curcumin Derivant and Tyrosinase by Fluorescence Quenching Method[J].Chinese Journal of Spectroscopy Laboratory,2011,28(4):1862-1865. |
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| Authors: | XU Xue-Tao ZHANG Rui-Rui DU Zhi-Yun LU Yu-Jing ZHANG Kun |
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| Institution: | XU Xue-Tao ZHANG Rui-Rui DU Zhi-Yun LU Yu-Jing ZHANG Kun(Faculty of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,P.R.China) |
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| Abstract: | Three structural stable curcumin derivant named 1,5-bis（4-Hydroxyphenyl）-1,4-pentadie-ne-3-one（1a）,2,5-bis（4-Hydroxybenzylidene）cyclopentanone（1b） and 2,6-bis（4-Hydroxyb-enzylidene）cyclohexanone（1c）with a monoketone were synthesized using bis-distyryl-ketone instead of bis-distyryl-bis-ketone.The interaction between curcumin derivant and tyrosinase was investigated by fluorescence quenching method.It was found that the fluorescence of compounds could be quenched by tyrosinase.The binding constant KA between tyrosinase and curcumin derivant were 104.8L·mol·^-1 for 1a,106.4L·mol^-1 for 1b,and 105.1L·mol^-1 for 1c with the binding sites（n） value of 0.95,1.21 and 1.05,respectively. |
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| Keywords: | Dienone Tyrosinase Fluorescence Spectrometry |
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