人工神经网络阻抑动力学光度法同时测定对苯二酚和邻苯二胺

在酸性介质中,F e（Ⅲ）能够催化H2O2氧化中性红褪色反应,而对苯二酚和邻苯二胺都能阻抑该催化氧化褪色反应的速度。但二者存在一定的速率差异,且吸光度的加和性不佳。结合人工神经网络提出了一种能同时测定对苯二酚和邻苯二胺的新方法。研究了最佳反应和测定条件,结果表明：1.0×10^-3m o.lL-1中性红溶液用量为0.8mL;30%H2O2用量为0.6mL;0.100g.L-1F e（Ⅲ）标准溶液用量为0.5mL;0.06m o.lL-1HC l用量为0.8mL;反应时间为6m in;反应温度为75℃时为最佳反应和测定条件。用于混合样品及河水中对苯二酚和邻苯二胺的测定,混合样品中对苯二酚和邻苯二胺回收率分别为95.6%—104.0%、97.8%—104.4%。河水中对苯二酚和邻苯二胺加标回收率均为105%,5次平行测定相对标准偏差分别为3.2%和2.5%。结果满意。

Simultaneous Determination of p-Dihydroxybenzene and o-Phenylenediamine by Inhibitory Kinetic Spectrophotometry with Artificial Neural Networks

In acidic medium,Fe（Ⅲ） could catalyze the reaction of H2O2 oxidation of neutral red fading,while p-dihydroxybenzene and o-phenylenediamine can inhibit the catalysis oxidation reaction rate of fading.But there was some obvious difference in their reaction rates.And their absorbance additive properties were not satisfactory.The new method for the simultaneous determination of p-dihydroxybenzene and o-phenylenediamine was established by inhibitory kinetics spectrophotometry with artificial neural network.The optimum conditions of reaction and determination were studied as follows：1.0×10^-3mol·L^-1 neutral red dosage of 0.8mL,30% H2O2 dosage of 0.6mL,standard solution Fe（Ⅲ）（0.100g·L^-1） of 0.5mL,0.06mol·L^-1HCl dosage of 0.8mL,reaction time of 6min and temperature of 75℃,that was applied to determination of p-dihydroxybenzene and o-phenylenediamine in mixed samples and water samples.The recovery of p-dihydroxybenzene and o-phenylenediamine in mixed samples were 95.6%—104.0% and 97.8%—104.4% respectively.The relative standard deviations of p-dihydroxybenzene and o-phenylenediamine were 3.2% and 2.5% for five replicate determinations.The recovery of standard addition in water sample for the method were both 105%.