Synthesis,characterization, antimicrobial,DNA binding,and oxidative cleavage activities of Cu(II) and Co(II) complexes with 2-(2-hydroxybenzylideneamino)isoindoline-1,3-dione |
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Abstract: | (E)-2-(2-hydroxybenzylideneamino)isoindoline-1,3-dione (Hbid) was prepared by condensation of N-aminophthalimide and salicylaldehyde and characterized by elemental analysis, IR, 1H-NMR, and mass spectral studies. Mononuclear complexes (phen)CuII(μ-Hbid)2H2O] (1), (phen)CoII(Cl)2(μ-Hbid)]6H2O (2) (phen?=?1,10-phenanthroline) and binuclear complexes CuII(μ-Hbid)]2 (3), and CoII(μ-Hbid)]2 (4) with Hbid were prepared and characterized by elemental analysis, IR, UV-Vis, molar conductance, and thermogravimetric (TG) techniques. DNA-binding properties of 1–4 were investigated by UV spectroscopy, fluorescence spectroscopy, and viscosity measurements. The results suggest that 1 and 2 bind to DNA by partial intercalation, whereas 3 and 4 find different groove-binding sites. The cleavage of these complexes with super coiled pUC19 has been studied using gel electrophoresis; all the complexes displayed chemical nuclease activity in the absence and presence of H2O2 via an oxidative mechanism. Complexes 1–4 inhibit the growth of both Gram-positive and Gram-negative bacteria. |
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Keywords: | 2-(2-Hydroxybenzylideneamino)isoindoline-1 3-dione Cu(II)/Co(II) complexes DNA binding Gel electrophoresis Antimicrobial activity |
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