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Control of column influence on the wide range pH gradient in ion-exchange chromatography |
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| Authors: | K Slais Z Friedl |
| Institution: | (1) Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Veve í 97, 611 42 Brno, Czechoslavakia;(2) Tessek Laboratory, Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Veveí 97, 611 42 Brno, Czechoslavakia |
| Abstract: | Summary A method for optimization of the mobile phase composition in ion exchange chromatography with a wide range external pH gradient (approx. over 5 pH units) has been formulated. It is shown that programming of various wide range pH profiles which are not impaired by buffer interactions of the mobile phase with sorbent is possible. Utilization of the wide range external pH gradient in cation-exchange chromatography of bovine haemoglobin is also demonstrated. |
| Keywords: | Ion-exchange chromatography External pH gradient Bovine haemoglobin |
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