EFFICIENT PHOTODYNAMIC ACTION OF VICTORIA BLUE BO AGAINST THE HUMAN LEUKEMIC CELL LINES K-562 AND TF-1 |
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| Authors: | Maciej Fiedorowicz Jean Remy Galindo Michel Julliard Patrice Mannoni Michel Chanon |
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| Institution: | Laboratoire d'Activation, Mécanime, Modélisation Moléculaire: AM3-URA CNRS 1411, Facultédes Sciences Saint Jérôme, 13397 Marseille Cedex 13, France;Department of Biology and INSERM U-119, Institute J. Paoli, I. Calmettes, 232 Boulevard Sainte Marguerite, 13009 Marseille, France |
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| Abstract: | Abstract— Photodynamic induced cytotoxicity by Victoria blue BO (VB-BO), merocyanine 540 (MC540), Nile blue A (NB) and 4-tetrasulfonatophenyl-porphyrin (4-TSPP) has been studied on two human leukemic cell lines: K-562 and TF-1. Cells were incubated with dyes and irradiated with different doses of white light. Cell survival was assessed by propidium iodide (PI) staining using flow cytometry analysis. Concentrations of 5 x 10 8 M VB-BO were found to kill 75% of cells, and a concentration of 1 × 10?7 M induced more than 99% of cell killing. To obtain the same cytotoxic level, the presence of 2.6 × 10?5 M of MCS40 during irradiation was needed. Under the conditions used, NB was ineflective as a photosensitizer, although uptake studies showed that this dye was taken by the cells in much greater amounts than any other studied dye. Cell cycle distribution of TF-1 cells, surviving MC540 or VB-BO photoscnsitization has bccn studied by flow cytometry analysis after staining with Hoechst 33342 and PI. It was found that cells in G1 phase were slightly more resistant toward MCS40– and VB-BO-mediated photosensitization than cells in other phascs of the ccll cycle |
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