TEMPERATURE DEPENDENCE OF INDUCTION OF CYCLOBUTANE-TYPE PYRIMIDINE PHOTODIMERS IN HUMAN FIBROBLASTS BY 313 nm LIGHT |
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| Authors: | Hugo J Niggli Peter A Cerutti |
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| Institution: | Department of Carcinogenesis, Swiss Institute for Experimental Cancer Research, CH-1066 Epalinges s/Lausanne, Switzerland |
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| Abstract: | The rate of cyclobutane-type pyrimidine photodimerization with 313 nm light was determined in confluent cultures of xeroderma pigmentosum cells of group A. A new method was developed for the determination of sodium borohydride reduced thymine-thymine (TT) and cytosine-thymine (CT) dirners by high pressure liquid chromatography. It was found that the yield of dimerization andtheratioof CT/TT depended on the irradiation temperature in the physiological fluence range of 2.25 to 15 kJ m−2. Both were significantly higher at 37 than at 0°C. |
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