Impact of ionic strength on adsorption capacity of chromatographic particles employed in separation of monoclonal antibodies |
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Authors: | Katarzyna Wrzosek Michal Grambli?ka Darina Tóthová Monika Anto?ová Milan Polakovi? |
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Institution: | 1.Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology,Slovak University of Technology,Bratislava,Slovak Republic |
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Abstract: | The influence of ionic strength on the adsorption capacity of seven commercial adsorbents used in downstream processing of
monoclonal antibodies was examined. Affinity (MabSelect, Poros 50A High Capacity, ProSep-vA High Capacity), hydrophobic charge-induction
(MEP HyperCel), and cation exchange adsorbents (FractoGel EMD SE Hicap (M), SP Sepharose Fast Flow, Ceramic HyperD F) were
used to study the adsorption of polyclonal human immunoglobulin G at optimal pH values. The ionic strength, adjusted by sodium
chloride concentrations in the range of 0–225 mM, strongly decreased the adsorption capacity of the cation exchangers. Equilibrium
data were described in the form of the dependence of the ratio of protein concentrations in the solid and liquid phases on
the total concentration of cation counter ions. They were successfully fitted and interpreted through a stoichiometric ion-exchange
model. |
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