Multi-modal applicability of a reversed-phase/weak-anion exchange material in reversed-phase,anion-exchange,ion-exclusion,hydrophilic interaction and hydrophobic interaction chromatography modes |
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Authors: | Email author" target="_blank">Michael?L?mmerhoferEmail author Raquel?Nogueira Wolfgang?Lindner |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Strasse 38, 1090 Vienna, Austria |
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Abstract: | We recently introduced a mixed-mode reversed-phase/weak anion-exchange type separation material based on silica particles
which consisted of a hydrophobic alkyl strand with polar embedded groups (thioether and amide functionalities) and a terminal
weak anion-exchange-type quinuclidine moiety. This stationary phase was designed to separate molecules by lipophilicity and
charge differences and was mainly devised for peptide separations with hydroorganic reversed-phase type elution conditions.
Herein, we demonstrate the extraordinary flexibility of this RP/WAX phase, in particular for peptide separations, by illustrating
its applicability in various chromatographic modes. The column packed with this material can, depending on the solute character
and employed elution conditions, exploit attractive or repulsive electrostatic interactions, and/or hydrophobic or hydrophilic
interactions as retention and selectivity increments. As a consequence, the column can be operated in a reversed-phase mode
(neutral compounds), anion-exchange mode (acidic compounds), ion-exclusion chromatography mode (cationic solutes), hydrophilic
interaction chromatography mode (polar compounds), and hydrophobic interaction chromatography mode (e.g., hydrophobic peptides).
Mixed-modes of these chromatographic retention principles may be materialized as well. This allows an exceptionally flexible
adjustment of retention and selectivity by tuning experimental conditions. The distinct separation mechanisms will be outlined
by selected examples of peptide separations in the different modes. |
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Keywords: | |
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