Separation of leucine and isoleucine by electrospray ionization-high field asymmetric waveform ion mobility spectrometry-mass spectrometry |
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| Authors: | David A. Barnett Barbara Ells Roger Guevremont Randy W. Purves |
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| Affiliation: | a Institute for National Measurement Standards, National Research Council of Canada, Ottawa, Ontario, Canada;b PE SCIEX, Concord, Ontario, Canada |
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| Abstract: | An electrospray ionization (ESI) source was used to generate gas-phase molecular anions of the amino acids leucine and isoleucine ((M–H)−; m/z −130), which were separated by high- field asymmetric waveform ion mobility spectrometry (FAIMS) and detected by quadrupole mass spectrometry (MS). This combination of ESI-FAIMS-MS enabled selective determination of either amino acid in mixtures that contained at least a 625-fold excess of the other. Comparisons with conventional ESI-MS showed a 50-fold improvement in the signal to background ratio for a 1 μM solution of leucine. |
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