| 雷公藤愈伤细胞悬浮培养生产雷公藤甲素的研究 |
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| 引用本文： | 朱留刚,封磊,洪伟,吴承祯,宋萍,李键,陈敏捷,黄鑫.雷公藤愈伤细胞悬浮培养生产雷公藤甲素的研究[J].北华大学学报(自然科学版),2011(6):708-712. |
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| 作者姓名： | 朱留刚 封磊 洪伟 吴承祯 宋萍 李键 陈敏捷 黄鑫 |
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| 作者单位： | 福建省高校森林生态系统过程与经营重点实验室;福建农林大学资源与环境学院;福建农林大学林学院; |
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| 基金项目： | 国家自然科学基金项目(30901131); 福建省科技计划重点项目(2009N0008); 福建省自然科学基金项目(2010J01062) |
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| 摘 要： | 对雷公藤叶愈伤组织进行悬浮培养,研究不同营养条件对雷公藤愈伤细胞生长及其雷公藤甲素质量分数的影响.结果表明：悬浮细胞在White培养基上增殖最快,而在MS培养基上生长最慢;White和MS培养基中悬浮细胞在第6天时雷公藤甲素质量分数最高,分别为7.14 mg/g和4.77 mg/g,而N6培养基中雷公藤甲素最大产量为6...
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| 关 键 词： | 雷公藤 愈伤组织 悬浮培养 甲素 |
Triptolide Production in Suspension-Cultured Tripterygium Wilfordii Cells |
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| ZHU Liu-gang,FENG Lei,HONG Wei,WU Cheng-zhen,SONG Ping,LI Jian,CHEN Min-jie,HUANG Xin.Triptolide Production in Suspension-Cultured Tripterygium Wilfordii Cells[J].Journal of Beihua University(Natural Science),2011(6):708-712. |
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| Authors: | ZHU Liu-gang FENG Lei HONG Wei WU Cheng-zhen SONG Ping LI Jian CHEN Min-jie HUANG Xin |
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| Institution: | ZHU Liu-gang1,FENG Lei2,HONG Wei1,3,WU Cheng-zhen1,SONG Ping1,LI Jian1,CHEN Min-jie1,HUANG Xin1(1.Higher Educational Key Laboratory for Forest Ecosystem Process and Management of Fujian Province,Fuzhou 350002,China,2.Resources and Environment College of Fujian Agriculture and Forestry University,3.Forestry College of Fujian Agriculture and Forestry University,China) |
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| Abstract: | The effects of nutritional conditions on cell growth and triptolide yield in Tripterygium wilfordii suspension culture were invested in this work.The resulted indicated that White medium was the best for the growth of suspension cells,while MS medium was the slowest.Both triptolide productions accumulation in Tripterygium cell in White and MS mediums reached the maximum yield at the sixth day,which were 7.14 mg/g and 4.77 mg/g respectively.The corresponding value in N6 medium was 6.34 mg/g,appeared at the n... |
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| Keywords: | Tripterygium wilfordii callus suspension culture triptolide |
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