Matrix-free laser desorption/ionization of ions landed on plasma-treated metal surfaces |
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Authors: | Volný Michael Sadílek Martin Jackson Karl E Diener Matthew Elam W Tim Turecek Frantisek |
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Institution: | Department of Chemistry, University of Washington, Seattle, WA, 98195-1700, USA. |
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Abstract: | We report new experiments in which laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF-MS) was applied to detection and characterization of gramicidin S and IgG pentapeptide (DSDPR) that were reactively landed on plasma-treated stainless steel surfaces. The distributions of M + H](+), M + Na](+) and M + K](+) ion species in LDI-TOF for gramicidin S and IgG pentapeptide (DSDPR) were found to be markedly different from those in conventional MALDI-TOF spectra of the same samples. LDI-TOF mass spectra showed a strong preference for M + K](+) adducts even in the presence of a large excess of sodium cations, or following surface treatment with trifluoroacetic acid. Alkali metal cations (K(+) and Cs(+)) can be exchanged in reactively landed peptide samples to provide the corresponding cationized peptide ions by LDI. Multiple charged trypsin cations were reactively landed into a layer of 2-(4-hydroxyphenylazo)benzoic acid and ionized by LDI. The ionization mechanisms for LDI of surface-deposited peptides are briefly discussed. Copyright (c) 2008 John Wiley & Sons, Ltd. |
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Keywords: | gramicidin S IgG pentapeptide laser desorption/ionization reactive landing soft‐landing surface enhanced Raman spectroscopy |
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