| 高效液相色谱法测定碳酸氢钠注射液中乙二胺四乙酸二钠 |
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| 引用本文： | 赵磊,刘北力,蒋黄卉,刘仕聪,郭思兰,孙艳涛.高效液相色谱法测定碳酸氢钠注射液中乙二胺四乙酸二钠[J].化学分析计量,2020,29(1):23-25. |
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| 作者姓名： | 赵磊 刘北力 蒋黄卉 刘仕聪 郭思兰 孙艳涛 |
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| 作者单位： | 四平市食品药品检验所,吉林四平136000;吉林省四平市第四人民医院,吉林四平136000;吉林师范大学化学学院,吉林四平136000;吉林省四平市妇婴医院,吉林四平136000 |
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| 摘 要： | 建立碳酸氢钠注射液中乙二胺四乙酸二钠的高效液相色谱测定方法。采用葡聚糖凝胶G-10色谱柱,以水为流动相,用高效液相色谱法测定碳酸氢钠注射液中乙二胺四乙酸二钠的含量。乙二胺四乙酸二钠的质量在0.1758~17.58μg的范围内与色谱峰面积线性关系良好,相关系数为0.9991,乙二胺四乙酸二钠的检出限为6.31μg/L。测定结果的相对标准偏差为0.89%(n=6),加标回收率为97.2%。该方法操作简单、准确、重现性好,可有效地控制碳酸氢钠注射液的质量。
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| 关 键 词： | 碳酸氢钠注射液 乙二胺四乙酸二钠 高效液相色谱法 |
Determination of disodium ethylenediamine tetraacetate in sodium bicarbonate injection by HPLC |
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| ZHAO Lei,LIU Beili,JIANG Huanghui,LIU Shicong,GUO Silan,SUN Yantao.Determination of disodium ethylenediamine tetraacetate in sodium bicarbonate injection by HPLC[J].Chemical Analysis And Meterage,2020,29(1):23-25. |
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| Authors: | ZHAO Lei LIU Beili JIANG Huanghui LIU Shicong GUO Silan SUN Yantao |
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| Institution: | (Siping City Institute for Food and Drug Control,Siping 136000,China;Fourth People's Hospital of Siping City,Siping 136000,China;Jilin Normal University College of Chemistry,Siping 136000,China;Siping Women and Children Hospital,Siping 136000,China) |
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| Abstract: | HPLC method for determining of disodium ethylenediamine tetraacetate in sodium bicarbonate injection was established.The content of disodium ethylenediamine tetraacetate was determined by using the glucan gel G-10 as a column and water as the mobile phase.The mass of disodium ethylenediamine tetraacetate had linear relation with the chromatograph area in the range of 0.1758-17.58μg with the correlation coefficient of 0.9991.The detection limit of isodium ethylenediamine tetraacetate was 6.31μg/L,the relative standard deviation of the detection results was 0.89%(n=6),and the standard addition recovery was 97.2%.The method is simple,accurate and reproducible,which can be used to control the quality of sodium bicarbonate injection. |
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| Keywords: | sodium bicarbonate injection isodium ethylenediamine tetraacetate HPLC |
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