接种量对MEC阳极膜形成及胞外聚合物的影响

本文采用单池微生物电解池(MEC)反应器,以活性污泥为接种物,葡萄糖为底物,研究了不同接种量对MEC阳极膜形成及胞外聚合物的影响。实验研究显示:10%、20%、30%和40%接种量下最大电流密度为0.315A·m^-2、5.94A·m^-2、12.28A·m^-2和0.64A·m^-2。表明,一定范围内增加接种量能提高阳极膜产电能力,接种量过多(达到40%)阳极膜产电能力反而下降。阳极生物膜电化学活性和氢气产率与MEC电流密度变化趋同,一定范围内增加接种量有利于提高阳极生物膜电化学活性和阳极膜氢气产率。进一步分析阳极膜生物量与胞外聚合物(EPS)成分结果表明:增加接种量有利于阳极膜生物量提高,大量的阳极附着细菌可以产生更高的电流密度和EPS含量,阳极膜EPS中蛋白质含量明显高于多糖,且随着蛋白质含量增加,产电密度也增大。

Effect of inoculum size on MEC anodic film formation and extracellular polymer

In this paper,the effects of different inoculations on the formation of MEC anode film and extracellular polymer were studied in a single cell microbial electrolysis tank(MEC)reactor with active sludge as inoculum and glucose as substrate.Experimental studies showed that the maximum current densities at 10%,20%,30%and 40%inoculations were 0.315A·m^-2,5.94 A·m^-2,12.28A·m^-2 and 0.64A·m^-2.The results showed that increasing the inoculum in a certain range could improve the power generation capacity of the anode film,but too much inoculum(up to 40%)decreased the power generation capacity of the anode film.The electrochemical activity and hydrogen production of anode biofilm were similar to the changes of MEC current density,and increasing the inoculum amount within a certain range was beneficial to improve the electrochemical activity and hydrogen production of anode biofilm.Further analysis of the anodic film composition of biomass and extracellular polymers(EPS)the results showed that the increase in quantity of make for improving anode membrane biomass,a large number of anode adherent bacteria could produce higher current density and EPS content,protein content in anode membrane EPS significantly higher than that of polysaccharides,and with the increase of protein content,electricity density also increased.