基于纳米银的检测黄曲霉毒素免疫传感器研究

黄曲霉毒素B1(AFB1)是食品中常见的强毒性霉菌毒素,如何对AFB1进行快速、准确、方便的检测受到了研究人员的广泛关注。本研究采用氧化石墨烯/纳米银/AFB1单克隆抗体复合材料(GO/Ag/AFB1抗体)修饰的金电极作为工作电极,利用纳米银良好的导电性实现较低的AFB1检出限。该免疫传感器在pH为7、温度为40℃、抗体与PBS体积比为1∶6000的最优条件下,无需添加信号放大物质,检出限最低可达到7.19×10^-10μg·kg^-1,检测范围为1.60×10^-9~1.68×10^-5μg·kg^-1,孵育时间为30s,回归方程为y=1.3101ln(x)+30.817(R^2=0.9916),测得含有AFB1实际玉米样品的回收率为91.42~107.00%。结果表明,GO/Ag/AFB1抗体免疫传感器具有在无需添加信号放大物质的情况下操作简便、响应迅速、灵敏度高、检出限低等特点,有望应用于食品领域AFB1的实时检测。

Immunosensor based on nano-silver for aflatoxin B1 detection

Aflatoxin B1(AFB1)is one of the most toxic mycotoxins in food contamination.Therefore,there is an urgent need to implement selective,sensitive and highly convenient methods for the determination of AFB1.A novel immunosensor modified by graphene oxide/nano-silver/AFB1 monoclonal antibody have been prepared.Based on the principle of the specific binding of AFB1 monoclonal antibody and antigen,the aflatoxin B1 was rapidly detected.The results shows that under the optimum conditions,the minimum detectable limit is 7.19×10^-10μg·kg^-1,the detection range is 1.60×10^-9~1.68×10^-5μg·kg^-1,and the equation is y=1.3101ln(x)+30.817(R^2=0.9916).The recovery rate of AFB1 in corn samples is in the range of 91.42~107.00%.The proposed immunosensor provides a new mode of simple operation,quick response,high sensitivity and low detection limit for AFB1 real-time detection in food industry.