Magnetic loading of tyrosinase-Fe3O4/mesoporous silica core/shell microspheres for high sensitive electrochemical biosensing

A new protocol is proposed for magnetic loading and sensitive electrochemical detection of phenol via the tyrosinase cross-linked mesoporous magnetic core/shell microspheres. The mesoporous magnetic microspheres, characterized by transmission electron microscopy, N(2) adsorption/desorption isotherms, and magnetic curve displays high capacity for enzyme immobilization and strong magnetism to adhere to the magnetic electrode surface without any additional adhesive reagent. The biosensor exhibits a wide linear response to phenol ranging from 1.0×10(-9) to 1.0×10(-5) M, a high sensitivity of 78 μA mM(-1), a low detection limit of 1 nM, and a fast response rate (less than 5s). The proposed method is simple, rapid, inexpensive and convenient in electrode renewal, which is recommended as a promising experimental platform for wider applications in biosensing.