CRAPT: an improved version of APT with compensation for variations in JCH

A modified version of the attached proton test (APT) sequence for ¹³C spectral editing, which we call CRisis‐APT (CRAPT), is developed and tested on representative organic compounds. CRAPT incorporates ¹³C compensation for refocusing inefficiency with synchronized inversion sweeps (CRISIS) pulses in combination with ¹H broadband inversion pulses to give improved compensation for variations in ¹J_CH along with improved refocusing efficiency. It is shown that CRAPT gives edited ¹³C spectra with only small losses in sensitivity (between 8% and 15% for strychnine, 1 , menthol, 2 , cholecalciferol, 3 , and isotachysterol, 4 ), compared with basic ¹³C spectra obtained on the same compounds. CRAPT also gives significantly better signal/noise than DEPTQ for nonprotonated carbons. Therefore, we conclude that CRAPT is an improvement over APT or DEPTQ or a combination of DEPT135 with a full ¹³C spectrum for routine ¹³C spectral editing of organic compounds. Copyright © 2014 John Wiley & Sons, Ltd.