Repair of (6-4) Photoproducts in Cultured Goldfish Cells at Confluence or Treated with H2O2 |
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| Authors: | Nobuhiro Uchida Hiroshi Mitani Akihiro Shima |
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| Institution: | Laboratory of Radiation Biology, Department of Biological Sciences, University of Tokyo, Tokyo, Japan;Laboratory of Experimental Radiology, Aichi Cancer Center Research Institute, Nagoya, Japan |
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| Abstract: | Previously we reported that fluorescent light (FL) illumination prior to UV irradiation (FL preillumination) had multiple effects on the repair of cyclobutane pyrim-idine dimers (CPD) and (6-4) photoproducts (6-4] PD) in cultured goldfish cells (RBCF-1) at the exponentially growing phase. In this study, it is shown that even under the confluent condition of RBCF-1 cells, FL preillumination increased the disappearance of (6-4) PD in the dark. In addition, both at confluence and at the exponentially growing phase, the disappearance of (6-4) PD after PR treatment was increased by FL preillumination to RBCF-1 cells. Moreover, it was found that H2O2 pre-treatment, followed by UV irradiation, of the exponentially growing cells also enhanced the disappearance of (6-4) PD in the dark and by photorepair treatment. The degree of enhancement by H2O2 pretreatment was almost the same as that by FL preillumination. |
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