Reversible gelatin-based hydrogels: Finetuning of material properties |
| |
Authors: | Sandra Van Vlierberghe Etienne Schacht Peter Dubruel |
| |
Institution: | Polymer Chemistry and Biomaterials Research Group, Ghent University, Krijgslaan 281 (Building S4), B-9000 Ghent, Belgium |
| |
Abstract: | The present work reports on the synthesis and evaluation of a crosslinkable thiolated gelatin derivative. The effect of varying two parameters including the pH of the reaction buffer and the thiolating agent applied (i.e. N-acetylhomocysteine thiolactone versus Traut’s reagent) on the obtained modification degree was studied in a first part. The gelatin derivatives synthesized starting from N-acetylhomocysteine thiolactone and Traut’s reagent were characterized in depth using size exclusion chromatography and UV–VIS spectrophotometry. In a subsequent part of the present work, hydrogel films were prepared starting from the thiolated gelatin derivative developed using N-acetylhomocysteine thiolactone. The contributions of both the chemical and the physical crosslinking of the hydrogels developed were studied in depth using rheology, swelling experiments and texturometry. The results indicate that the physical structuring, inherent to gelatin, contributes to a large extent to the mechanical properties. However, the chemical crosslinking mostly determines the final hydrogel properties and can be controlled to a large extent. The gelatin-based gels are flexible, strong and transparent. A major advantage of disulfide-crosslinked hydrogels is the fact that the crosslinking is reversible. The latter could be interesting in view of future applications as cell carriers for tissue engineering. |
| |
Keywords: | Gelatin modification Thiolation Physical crosslinking Chemical crosslinking Hydrogel properties |
本文献已被 ScienceDirect 等数据库收录! |
|