Determination of cyanide exposure by gas chromatography-mass spectrometry analysis of cyanide-exposed plasma proteins |
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Authors: | Stephanie L Youso John P Lee |
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Institution: | a Department of Chemistry and Biochemistry, South Dakota State University, Shepard Hall 121, Box 2202, Brookings, SD 57007, USA b US Army Medical Research Institute of Chemical Defense, 3100 Ricketts Point Road, Aberdeen Proving Ground, MD 21010-5400, USA |
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Abstract: | Exposure to cyanide can occur in a variety of ways, including exposure to smoke from cigarettes or fires, accidental exposure during industrial processes, and exposure from the use of cyanide as a poison or chemical warfare agent. Confirmation of cyanide exposure is difficult because, in vivo, cyanide quickly breaks down by a number of pathways, including the formation of both free and protein-bound thiocyanate. A simple method was developed to confirm cyanide exposure by extraction of protein-bound thiocyanate moieties from cyanide-exposed plasma proteins. Thiocyanate was successfully extracted and subsequently derivatized with pentafluorobenzyl bromide for GC-MS analysis. Thiocyanate levels as low as 2.5 ng mL−1 and cyanide exposure levels as low as 175 μg kg−1 were detected. Samples analyzed from smokers and non-smokers using this method showed significantly different levels of protein-bound thiocyanate (p < 0.01). These results demonstrate the potential of this method to positively confirm chronic cyanide exposure through the analysis of protein-bound cyanide in human plasma. |
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Keywords: | Thiocyanate Protein adducts Biomarker Gas chromatography-mass spectrometry Extraction Bioanalysis Derivatization |
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