Polarized triplet-minus-singlet absorbance difference spectra measured by absorbance-detected magnetic resonance. An application to photosynthetic reaction centres |
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| Authors: | HJ den Blanken RF Meiburg AJ Hoff |
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| Institution: | Centre for the Study of the Excited States of Molecules and Department of Biophysics, Huygens Laboratory, State University of Leyden, Leyden, The Netherlands |
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| Abstract: | A method is presented to measure linear dichroic triplet-minus-singlet absorbance difference spectra using absorbance-detected magnetic resonance of the triplet state in zero magnetic field. Orientational selection is achieved by using a linearly-polarised microwave field. The microwave-induced change in the absorbance of light with the electric vector parallel or perpendicular to the microwave field vector is monitored using a Morvue photoelastic modulator and an analyzing polarizer. The method is applied to reaction centres of the photosynthetic bacterium Rhodopseudomonas viridis. |
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