| WHOLE-CELL CLAMP STUDY OF XENOPUS EMBRYONIC CHOLINERGIC NEURONS |
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| 作者姓名： | 谢佐平 王堤 竺煜杰 |
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| 作者单位： | Department of Biology，Tsinghua University，Beijing，Department of Biology，Tsinghua University，Beijing，Department of Biology，Tsinghua University，Beijing |
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| 基金项目： | Project supported by the National Natural Science Foundation of China |
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| 摘 要： | Whole-cell clamped myoballs are placed into direct visible contact with the growth cones of isolated neurons in embryonic Xenopus culture to serve as probe of acetylchollne (AcCHo) release in order to determine whether these neurons are cholinergic or not. Using a GQ-seal, whole-cell recording method, the electrophysiological properties of these identified cholinergic neurons are studied. It is found that these embryonic neurons, like adult frog motor neurons, exhibit repetitive firings in a certain embryonic developing stage. A development of repetitive firings is observed simultaneously. Tracing the development of one neuron, we find that the development of repetitive firing is completed at the 48th h after fertilization. Tetrodotoxin (TTX) which blocks Na~+ channels can abolish all firings; and tetraethyl ammonium chloride (TEA), the blocker of K~+ channels, reverses this development, i. e. it makes the repetitive firings disappear again. These data show that the nature of the development of repetiti
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WHOLE-CELL CLAMP STUDY OF XENOPUS EMBRYONIC CHOLINERGIC NEURONS |
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| XIE ZUO-PING WANG TI AND ZHU YU-JIE.WHOLE-CELL CLAMP STUDY OF XENOPUS EMBRYONIC CHOLINERGIC NEURONS[J].Science in China(Chemistry),1989(2). |
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| Authors: | XIE ZUO-PING WANG TI AND ZHU YU-JIE |
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| Abstract: | Whole-cell clamped myoballs are placed into direct visible contact with the growth cones of isolated neurons in embryonic Xenopus culture to serve as probe of acetylchollne (AcCHo) release in order to determine whether these neurons are cholinergic or not. Using a GQ-seal, whole-cell recording method, the electrophysiological properties of these identified cholinergic neurons are studied. It is found that these embryonic neurons, like adult frog motor neurons, exhibit repetitive firings in a certain embryonic developing stage. A development of repetitive firings is observed simultaneously. Tracing the development of one neuron, we find that the development of repetitive firing is completed at the 48th h after fertilization. Tetrodotoxin (TTX) which blocks Na~+ channels can abolish all firings; and tetraethyl ammonium chloride (TEA), the blocker of K~+ channels, reverses this development, i. e. it makes the repetitive firings disappear again. These data show that the nature of the development of repetitive firings is the development of K~+ channels. |
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| Keywords: | whole-cell clamp cholinergic neurous repetitive firings Xenopus embryo |
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