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杀虫剂莠去津免疫传感器功能膜的制备与表征
引用本文:李文霞,杜振霞,陈朝晖.杀虫剂莠去津免疫传感器功能膜的制备与表征[J].光谱实验室,2005,22(4):876-880.
作者姓名:李文霞  杜振霞  陈朝晖
作者单位:北京服装学院北京市重点实验室,北京市朝阳区樱花东路甲2号604信箱,100029;北京化工大学理学院分析测试中心,北京市,100029;北京金源化学集团有限公司研发部,北京市,100029
摘    要:用杀虫剂莠去津标记聚乙烯胺,然后共价偶联到已自组装在金基板上的11-巯基十一酸单分子层上,形成杀虫剂莠去津功能化的表面。利用抗体抗原相互特异性作用,将ATZ抗体固定在功能化金基板的表面。功能化的表面通过FT-IRRAS进行了表征,对功能表面的形成特性以及与抗体的相互作用情况进行了研究。

关 键 词:痕量杀虫剂传感器  傅里叶变换红外反射吸收光谱  莠去津  自组装
文章编号:1004-8138(2005)04-0876-05
收稿时间:2005-06-06
修稿时间:2005年6月6日

The Preparation and Characterization of Function Membrane of a Novel Immunosensor Detector for Pesticides Atrazine
LI Wen-xia,DU Zheng-Xia,CHEN Zhao-Hui.The Preparation and Characterization of Function Membrane of a Novel Immunosensor Detector for Pesticides Atrazine[J].Chinese Journal of Spectroscopy Laboratory,2005,22(4):876-880.
Authors:LI Wen-xia  DU Zheng-Xia  CHEN Zhao-Hui
Abstract:Taking advantage of pesticides atrazine marked polyamine, followed by covalent coupling to a self-assembled monolayer of 11-mercaptoundecanoic acid in the gold substrate, pesticides atrazine functionalized membrane was formed. Utilizing the specific interaction between the antigen and the antibody, we have successfully attached atrazine antibody to functionalized gold substrate membrane. The functionalized surfaces are characterised by FT-IRRAS, the formation property of function membrane and the interaction between it and atrazine antibody was studied.
Keywords:Trace Pesticides Detector  FT-IRRAS  Atrazine  Self-Assembled
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