| 3种苯丙胺类药物与溶菌酶的作用机制及构效关系研究 |
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| 引用本文： | 张爱平,黄茜,郝娟,文雯,高晓亚. 3种苯丙胺类药物与溶菌酶的作用机制及构效关系研究[J]. 分析测试学报, 2011, 30(2): 140-145. DOI: 10.3969/j.issn.1004-4957.2011.02.005 |
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| 作者姓名： | 张爱平 黄茜 郝娟 文雯 高晓亚 |
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| 作者单位： | 山西医科大学药学院; |
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| 基金项目： | 山西省自然科学基金资助项目(2010011048-1); 山西医科大学科技创新基金资助项目(01200806) |
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| 摘 要： | 在模拟人体生理条件下,采用荧光光谱法研究了3种不同结构的苯丙胺类药物(麻黄碱、伪麻黄碱和甲基麻黄碱)与溶菌酶之间的相互作用,计算了其结合常数、结合位点数和热力学参数,并探讨了3种药物对溶菌酶构象的影响.研究发现,三者可对溶菌酶内源性荧光产生强烈的猝灭作用,其猝灭过程均为静态猝灭.麻黄碱、伪麻黄碱和甲基麻黄碱与溶菌酶均形...
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| 关 键 词： | 麻黄碱 伪麻黄碱 甲基麻黄碱 溶菌酶 荧光光谱法 同步荧光光谱法 |
Study on Reaction Mechanism and Their Structure-Activity Relationship between Three Amphetamines and Lysozyme |
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| ZHANG Ai ping,HUANG Qian,HAO Juan,WEN Wen,GAO Xiao ya. Study on Reaction Mechanism and Their Structure-Activity Relationship between Three Amphetamines and Lysozyme[J]. Journal of Instrumental Analysis, 2011, 30(2): 140-145. DOI: 10.3969/j.issn.1004-4957.2011.02.005 |
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| Authors: | ZHANG Ai ping HUANG Qian HAO Juan WEN Wen GAO Xiao ya |
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| Affiliation: | ZHANG Ai-ping,HUANG Qian,HAO Juan,WEN Wen,GAO Xiao-ya(Department of Pharmacy,Shanxi Medical University,Taiyuan 030001,China) |
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| Abstract: | The interactions of lysozyme with ephedrine,pseudoephedrine and methylephedrine,as well as their structure-activity relationship were investigated by fluorescence spectrometry under simulative physiological conditions.The binding constant,the number of binding sites and the thermodynamic parameters were calculated and the effects of ephedrine,pseudoephedrine and methylephedrine on the conformation of lysozyme were studied.The results showed that the endogenous fluorescence of lysozyme was significantly quen... |
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| Keywords: | ephedrine pseudoephedrine methylephedrine lysozyme fluorescence spectroscopy synchronous fluorescence spectroscopy |
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