水光谱探针及其在结构分析中的应用

水对化学和生物过程具有重要作用,因此水的结构和性质研究一直备受关注。水的结构易受温度影响,利用温控近红外光谱技术和化学计量学方法,通过提取随温度变化的水光谱信息,不仅可以了解水的结构和性质,还可以将水作为探针,探测溶液或生物体系中分子的定量信息和结构变化。该文总结了利用温控近红外光谱技术研究小分子的结构和蛋白质、温敏聚合物结构转变过程等方面的研究工作,并利用随温度变化的水光谱信息,对化学结构及其变化过程中水的作用及作用机理进行了分析。

Water as a Spectroscopic Probe for Detection of Structural Analysis

Water plays an important role in chemical and biological processes,attracting wide interest in the structure of water.Based on the effect of temperature on structure of water,a temperature dependent near infrared spectroscopy was proposed and applied to the quantitative and structural analysis of aqueous solutions.Meanwhile,chemometrics was adopted to extract the temperature induced spectral variation of the water.In this article,recent works on the structural analysis of small molecules and the structural transformation of proteins and thermo responsive polymers were summarized.The structural changes and the interactions of them in aqueous solutions,as well as the role of water in the chemical processes were studied using the spectral variation of water with temperature.