Applying microdroplets as sensors for label-free detection of chemical reactions |
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Authors: | Hofmann Tobias W Hänselmann Siegfried Janiesch Jan-Wilhelm Rademacher Anne Böhm Christian H J |
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Institution: | Max Planck Institute for Intelligent Systems, Department of New Materials and Biosystems, Stuttgart, Germany. |
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Abstract: | Despite its tremendous high-throughput screening capabilities, widespread applications of droplet-based microfluidics are still limited by the poor availability of appropriate analytical assays. Here we report on a novel sensor method that exploits the osmosis-driven change in droplet size as a quantitative and label-free marker for reactions inside the droplets. We present an analysis of the underlying mechanism and apply the method for monitoring metabolic activity at a single-cell level. |
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