Automated analytical microarrays: a critical review |
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Authors: | Michael Seidel Reinhard Niessner |
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Institution: | 1.Chair for Analytical Chemistry and Institute of Hydrochemistry,Technische Universit?t München,München,Germany |
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Abstract: | Microarrays provide a powerful analytical tool for the simultaneous detection of multiple analytes in a single experiment.
The specific affinity reaction of nucleic acids (hybridization) and antibodies towards antigens is the most common bioanalytical
method for generating multiplexed quantitative results. Nucleic acid-based analysis is restricted to the detection of cells
and viruses. Antibodies are more universal biomolecular receptors that selectively bind small molecules such as pesticides,
small toxins, and pharmaceuticals and to biopolymers (e.g. toxins, allergens) and complex biological structures like bacterial
cells and viruses. By producing an appropriate antibody, the corresponding antigenic analyte can be detected on a multiplexed
immunoanalytical microarray. Food and water analysis along with clinical diagnostics constitute potential application fields
for multiplexed analysis. Diverse fluorescence, chemiluminescence, electrochemical, and label-free microarray readout systems
have been developed in the last decade. Some of them are constructed as flow-through microarrays by combination with a fluidic
system. Microarrays have the potential to become widely accepted as a system for analytical applications, provided that robust
and validated results on fully automated platforms are successfully generated. This review gives an overview of the current
research on microarrays with the focus on automated systems and quantitative multiplexed applications.
Figure MCR 3: A fully automated chemiluminescence microarray reader for analytical microarrays |
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Keywords: | Analytical microarrays Biosystems technology Environmental analysis Food analysis Clinical diagnostics |
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