Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling |
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Authors: | J -P Vietzke M Straßner U Hintze |
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Institution: | 1. Institut für Organische Chemie, Universit?t Hamburg, Martin-Luther-King Platz 6, 20146, Hamburg, Germany 2. Department of Analytical Research, Beiersdorf AG, Kst. 4231, Unnastra?e 48, 20253, Hamburg, Germany
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Abstract: | Summary A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling
of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray
ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation.
Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra
from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.
Summary A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling
of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray
ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation.
Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra
from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography. |
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Keywords: | Column liquid chromatography Electrospray ionization mass spectrometry MS-MS ion-trap experiments Human stratum corneum ceramides |
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