Development and validation of liquid chromatography–mass spectrometry method for the determination of telmisartan in human plasma |
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Authors: | Ben-mei Chen Yi-zeng Liang Ya-li Wang Fu-Liang Deng Ping Zhou Fang-qiu Guo Lan-fang Huang |
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Institution: | aAnalytical Testing Center of Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, China bResearch Center of Modernization of Chinese Medicine, College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, China |
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Abstract: | A sensitive liquid chromatographic–electrospray ionization mass spectrometric method was developed and validated for fast determination of telmisartan in human plasma. Plasma of 0.1 mL was deprotienated with methanol, centrifugation, evaporation to dryness and dissolving in mobile phase, samples were separated using a Hypersil-Keystone C18 reversed-phase column (150 mm × 2.1 mm i.d., 5 μm), together with a mobile phase containing of acetonitrile–10 mM ammonium acetate (42:58, v/v), 0.2% acetic acid and was isocratically eluted at a flow rate of 0.2 mL/min. Telmisartan and its internal standard, valsartan, were measured by electrospray ion source in positive selective ion monitoring mode. The method demonstrated linearity from 1 to 2000 ng/mL (r = 0.9988). The limit of quantification for telmisartan in plasma was 1 ng/mL with good accuracy and precision. The mean sample extract recovery of the method were higher than 82 and 78% for telmisartan and internal standard (IS), respectively. The within-run and between-run precision ranged from 3.4 to 8.9% and 5.9 to 11.2% (relative standard deviation, R.S.D.), respectively. |
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Keywords: | Telmisartan LC–MS Electrospray ionization |
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