Liposomes-entrapped chondroitin sulphate: ultrastructural characterization and in vitro biocompatibility |
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Authors: | Trif Mihaela Moldovan Lucia Moisei Magdalena Craciunescu Oana Zarnescu Otilia |
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Affiliation: | Institute of Biochemistry, Splaiul Independentei 296, 060031 Bucharest, Romania. |
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Abstract: | The purpose of this study was ultrastructural characterization of liposomes-entrapped chondroitin sulphate and to prove their in vitro biocompatibility in a human dermal fibroblast culture system, in order to use liposome-entrapped chondroitin sulphate in the treatment of inflammatory disorders. Chondroitin sulphate entrapped in liposomes appears as electron-dense particles in ultra-thin section. Comparative studies using chondroitin sulphate, empty liposomes and liposome-chondroitin sulphate systems were performed in order to evaluate their effect on growth and morphology of fibroblasts after 48 h of culture. Light microscopy indicated that chondroitin sulphate, empty liposomes and liposome-chondroitin sulphate systems do not induce appreciable cytotoxic effects, and cells maintain normal morphology when compared to control fibroblasts. |
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