Bubble cell for magnetic bead trapping in capillary electrophoresis |
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Authors: | Gassner Anne-Laure Proczek Gaëlle Girault Hubert H |
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Affiliation: | (1) Laboratoire d’Electrochimie Physique et Analytique, Ecole Polytechnique F?d?rale de Lausanne, EPFL SB ISIC LEPA, Station 6, 1015 Lausanne, Switzerland; |
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Abstract: | A bubble cell capillary classically used to extend the optical path length for UV–vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case with magnetic beads trapped in a standard capillary, thereby having less effect on the experimental conditions. Using numerical simulations and microscopic visualizations, the capture of beads inside a bubble cell was investigated with two magnet configurations. Pressure-driven and electro-osmotic flow velocities were measured for different amounts of protein-A-coated beads or C18-functionalized beads (RPC-18). Solid-phase extraction of a model antibody on protein-A beads and preconcentration of fluorescein on RPC-18 beads were performed as proof of concept experiments. |
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