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IDENTIFICATION OF THE EXOPOLYSACCHARIDE AMYLOVORAN BY NMR[1]
Abstract:Structural analysis of the exopolysaccharide (EPS) amylovoran produced by different natural Erwinia amylovora isolates revealed repeating pentasaccharide substructures substituted 30–40% with a sixth monosaccharide when isolated from host plants of the Malaceae species. Only a pentasaccharide substructure was found for pathogens isolated from Rubus plants. The differences between both substructures, obtained after treatment of the amylovorans with a depolymerase, were shown with NMR. The host range of fire blight bacteria could be partially found in this difference.
Keywords:Fucoidan fragments  Synthesis  NMR  Conformational analysis  NOE
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