| Abstract: | A kind of macroporous bead carrier of copolymer containing monomer units of N-aminoethyl acrylamide and vinylalcohol was synthesized, i.e. the MR-AA carrier. Papain was immobilized on the carrier using glutaraldehyde as the couplingagent. The enzymatic activity of the immobilized papain was compared with free papain using casein as a substrate, and theeffects of glutaraldehyde concentration, pH, temperature, time and papain amount added on the activity recovery were alsoinvestigated. The results show that the MR-AA carrier contains reactive primary amine groups,hydrophilic amido links and hydroxyl groups,as well as macroporous structures based on its matrix (MR-AV matrix),furthermore,the activity recovery of papain in the immobilization could reach 48%～58%.In comparison with free papain,the resulting immobilized papain exhibits a remarkable thermostability and better reusability. |
