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Microcontact printing of multiproteins on the modified mica substrate and study of immunoassays
Abstract:Microcontact printing (µCP) is an easy and efficient way of producing patterns of self‐assembled monolayers (SAM) containing different functional groups. We have developed a simple and convenient µCP‐based technique for the modification of a mica substrate with 3‐aminopropyltriethoxysilane (APTES) and the micropatterning of proteins (chicken IgG and rabbit IgG) on the modified mica surface. Our approach provides a quick and easy way to produce protein patterns on solid surfaces. The printed immunoglobulin patterns were detected by exposing the substrate to solutions containing fluorescently labeled complementary anti‐IgGs, and the formed immunoassays were studied using fluorescence microscopy. Copyright © 2005 John Wiley & Sons, Ltd.
Keywords:microcontact printing (µ  CP)  surface modification  mica  immunoassays
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